Heartland Assays

Amino Acids, HMB and Additional Services

Heartland Assays offers several other analytical services aside from Vitamin D and 3-methylhistidine analysis. These services include physiological amino acid analysis, β-hydroxy-β-methylbutyrate (HMB) analysis, hormone analysis (multiple), protein assays, curcumin and curcuminoid metabolite analysis, colorimetric assays, and creatinine, calcium, magnesium and phosphorus analysis.


Amino Acid Analysis by  LC/MS/MS

Heartland Assays offers amino acid analysis for free amino acids by either liquid chromatography/tandem mass spectrometry (LC/MS/MS) using the EZ: faast™ procedure. This procedure consists of a solid phase extraction followed by derivatization and liquid/liquid extraction. This service is available for plasma/serum samples.


β-Hydroxy-β-methylbutyrate (HMB) Analysis

The lab has had extensive experience working with HMB analysis from previous research conducted by the company. HMB is an active metabolite of the amino acid leucine and has the ability to suppress proteolysis while it minimizes muscle damage.1 HMB is sold as a nutritional supplement to increase strength, improve endurance, and reduce recovery time. HMB analysis is available for plasma/serum or urine samples.

Hormone, Peptide, and Protein Analysis*

Hormone analysis by enzyme-linked immunosorbent assay (ELISA) technique can be performed in the lab. Examples of hormones that can be analyzed include: IGH-1, IGFBP-3 (total), IGF-1, cortisol, DHBA, testosterone, C-reactive protein, insulin, C-peptide of insulin. Other hormones can be analyzed upon request.
*Analysis offered for research purposes only


Protein Assays

Protein assays can be done on various samples using the BCA (bicinchoninic acid) Protein Assay.


Curcumin and Curcuminoid Metabolite Analysis

Curcuminoids are measured by liquid chromatography/tandem mass spectrometry (LC/MS/MS).


Colorimetric Assays


Creatinine, Calcium, Magnesium and Phosphorus Analysis



Works Cited

  1. Wilson G.J., Wilson J.M. and Manninen A.H. 2008. Effects of beta-hydroxy-beta-methylbutyrate (HMB) on exercise performance and body composition across varying levels of age, sex, and training experience: A review. Nutr. Metab. (Lond) 5: 1. View Abstract


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